Determination of pyridoxine by means of an indicator diazo-test strip

Vera M Ostrovskaya, Alexander V Ivanov* and Dmitry Yu Marchenko

Pyridoxine (vitamin B6) plays an important role in the body’s metabolism. Reaction of azocoupling with diazoaryl compound underlies in a basis a known photometric method of its chemical analysis. This method is not selective. In the present work the test method of determination of the pyridoxine containing in a molecule hydroxyarylgroup, was proposed by them azocoupling with chromogenic indicator diazo test strip, consisting of a covalently fixed on cellulose reagent with functional diazoaryl group, attached to the cellulose carrier through 1,3-diphenoxypropane bridge. This test strip interaction with resulted in formation of cellulose tetradentate chelating agent, capable to enter the subsequent chromogenic reaction with copper (II) ion. Formation of blue-violet complex of azoderivative pyridoxine with copper was used for determination of the content of pyridoxine (vitamin B6) in a number of his pharmaceutical preparations.

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Published on: Jun 28, 2019 Pages: 1-5

Full Text PDF Full Text HTML DOI: 10.17352/ojabc.000005
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